Composite
Part:BBa_K3027004:Design
Designed by: Arnaud Boudigou Group: iGEM19_GO_Paris-Saclay (2019-10-16)
Arabinose-inducible nuclease_A1 expression module
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1216
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1216
Illegal NheI site found at 1205 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1216
Illegal BamHI site found at 1144 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1216
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1216
Illegal AgeI site found at 979 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Since we used Golden Gate assembly, we made sure that our parts were devoid of BsaI and SapI sites.
Source
A1 is a nuclease coming from bacteriophage T5.